High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Description:
Anti-Mouse IgG&Rabbit IgG for WB, AlpSdAbs® VHH(HRP) is designed for detecting both mouse IgG and rabbit IgG in western blot experiment specifically. Anti-Mouse IgG&Rabbit IgG for WB, AlpSdAbs® VHH(HRP) is based on recombinant single domain antibodies to mouse IgG(H+L) and rabbit IgG(H+L) coupled to HRP. Based on immunoelectrophoresis and/or ELISA, Anti-Mouse IgG&Rabbit IgG for WB, AlpSdAbs® VHH(HRP) detects the mouse IgG and rabbit IgG, no reactivity with human, cynomolgus, rat, goat IgG.
Immunogen: Recombinant mouse IgG/rabbit IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: HRP
Specificity: Mouse IgG&Rabbit IgG
Cross-Reactivity: No cross-reactivity with human, cynomolgus, rat, goat IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/mL
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles), Protect from light.
Background:
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
WB: 1:10000-1:50000
WB: 1ul Nano-secondary antibody for 1ul First antibody
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
